Western Blot:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: FKBP5 #12210 Cell Signaling Technology - validated by WB using HT-1080 and Hela cells (CST website) ACC #3676 Cell Signaling Technology - validated by WB using 3T3, 293 and various cell lines (CST website) ACLY #4332 Cell Signaling Technology - validated by WB using Hela, 3T3, C6 and COS cells (CST website) p-ACLY #4331 Cell Signaling Technology - validated by WB using PDGF-treated 3T3 cells (CST website) PKM1/2 #3190 Cell Signaling Technology - validated by WB using MCF-7, HepG2 and various cell lines (CST website) GLUT1 #12939 Cell Signaling Technology - validated by WB using RD, Huh6, 3T3 and A204 cells (CST website) AMPKa #2603 Cell Signaling Technology - validated by WB using 293, COS, PC12 cells and mouse brain extracts (CST website) p-AMPKa #2535 Cell Signaling Technology - validated by WB using oligomycin-treated C2C12 cells (CST website) BIP #3177 Cell Signaling Technology - validated by WB using A204, L929, U-87MG (CST website) DNAJC3 #2940 Cell Signaling Technology - validated by WB using 3T3, Hepal, bTC6 and NIT-1 cells (CST website) XBP1s #40435 Cell Signaling Technology - validated by WB using tunicamycin-treated 293T cells (CST website) CHOP #2895 Cell Signaling Technology - validated by WB using thapsigargin treated C6 and A204 cells (CST website) EPCAM #93790 Cell Signaling Technology - validated by WB using HCT116,HT-29 and various cell lines (CST website) ATF4 #11815 Cell Signaling Technology - validated by WB using tunicamycin-treated 293 and Hela cells (CST website) PARP #9532 Cell Signaling Technology - validated by WB using 293 PARP KO cells (CST website) DECR1 ab95965 Abcam - validated by WB using HepG2 cells (CST website) GPX4 ab125066 Abcam - validated by WB using mouse and rats testis lysates AR ChIP grade #5153 Cell Signaling Technology - validated by CHiP on LNCaP cells grown in phenol red free medium and 5% charcoal stripped FBS for 3 d then treated with dihydrotestosterone (DHT, 10 nM) for 4 hours (CST website) AR ChIP grade 17-10489 Millipore - validated by CHiP on DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Millipore website) All antibodies have been validated for WB on human cell lines in this study (clear band at the right Mw) AR ChIP antibodies have been validated using CHiP on LNCaP cells (KLK3 as a positive control) Eukaryotic cell lines Policy information about cell lines Cell line source(s) LNCaP, CWR22Res, VCap and C4-2 cells were purchased from ATCC.
Chromatin Immunoprecipitation:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: FKBP5 #12210 Cell Signaling Technology - validated by WB using HT-1080 and Hela cells (CST website) ACC #3676 Cell Signaling Technology - validated by WB using 3T3, 293 and various cell lines (CST website) ACLY #4332 Cell Signaling Technology - validated by WB using Hela, 3T3, C6 and COS cells (CST website) p-ACLY #4331 Cell Signaling Technology - validated by WB using PDGF-treated 3T3 cells (CST website) PKM1/2 #3190 Cell Signaling Technology - validated by WB using MCF-7, HepG2 and various cell lines (CST website) GLUT1 #12939 Cell Signaling Technology - validated by WB using RD, Huh6, 3T3 and A204 cells (CST website) AMPKa #2603 Cell Signaling Technology - validated by WB using 293, COS, PC12 cells and mouse brain extracts (CST website) p-AMPKa #2535 Cell Signaling Technology - validated by WB using oligomycin-treated C2C12 cells (CST website) BIP #3177 Cell Signaling Technology - validated by WB using A204, L929, U-87MG (CST website) DNAJC3 #2940 Cell Signaling Technology - validated by WB using 3T3, Hepal, bTC6 and NIT-1 cells (CST website) XBP1s #40435 Cell Signaling Technology - validated by WB using tunicamycin-treated 293T cells (CST website) CHOP #2895 Cell Signaling Technology - validated by WB using thapsigargin treated C6 and A204 cells (CST website) EPCAM #93790 Cell Signaling Technology - validated by WB using HCT116,HT-29 and various cell lines (CST website) ATF4 #11815 Cell Signaling Technology - validated by WB using tunicamycin-treated 293 and Hela cells (CST website) PARP #9532 Cell Signaling Technology - validated by WB using 293 PARP KO cells (CST website) DECR1 ab95965 Abcam - validated by WB using HepG2 cells (CST website) GPX4 ab125066 Abcam - validated by WB using mouse and rats testis lysates AR ChIP grade #5153 Cell Signaling Technology - validated by CHiP on LNCaP cells grown in phenol red free medium and 5% charcoal stripped FBS for 3 d then treated with dihydrotestosterone (DHT, 10 nM) for 4 hours (CST website) AR ChIP grade 17-10489 Millipore - validated by CHiP on DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Millipore website) All antibodies have been validated for WB on human cell lines in this study (clear band at the right Mw) AR ChIP antibodies have been validated using CHiP on LNCaP cells (KLK3 as a positive control) Eukaryotic cell lines Policy information about cell lines Cell line source(s) LNCaP, CWR22Res, VCap and C4-2 cells were purchased from ATCC.
Stable Transfection:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: FKBP5 #12210 Cell Signaling Technology - validated by WB using HT-1080 and Hela cells (CST website) ACC #3676 Cell Signaling Technology - validated by WB using 3T3, 293 and various cell lines (CST website) ACLY #4332 Cell Signaling Technology - validated by WB using Hela, 3T3, C6 and COS cells (CST website) p-ACLY #4331 Cell Signaling Technology - validated by WB using PDGF-treated 3T3 cells (CST website) PKM1/2 #3190 Cell Signaling Technology - validated by WB using MCF-7, HepG2 and various cell lines (CST website) GLUT1 #12939 Cell Signaling Technology - validated by WB using RD, Huh6, 3T3 and A204 cells (CST website) AMPKa #2603 Cell Signaling Technology - validated by WB using 293, COS, PC12 cells and mouse brain extracts (CST website) p-AMPKa #2535 Cell Signaling Technology - validated by WB using oligomycin-treated C2C12 cells (CST website) BIP #3177 Cell Signaling Technology - validated by WB using A204, L929, U-87MG (CST website) DNAJC3 #2940 Cell Signaling Technology - validated by WB using 3T3, Hepal, bTC6 and NIT-1 cells (CST website) XBP1s #40435 Cell Signaling Technology - validated by WB using tunicamycin-treated 293T cells (CST website) CHOP #2895 Cell Signaling Technology - validated by WB using thapsigargin treated C6 and A204 cells (CST website) EPCAM #93790 Cell Signaling Technology - validated by WB using HCT116,HT-29 and various cell lines (CST website) ATF4 #11815 Cell Signaling Technology - validated by WB using tunicamycin-treated 293 and Hela cells (CST website) PARP #9532 Cell Signaling Technology - validated by WB using 293 PARP KO cells (CST website) DECR1 ab95965 Abcam - validated by WB using HepG2 cells (CST website) GPX4 ab125066 Abcam - validated by WB using mouse and rats testis lysates AR ChIP grade #5153 Cell Signaling Technology - validated by CHiP on LNCaP cells grown in phenol red free medium and 5% charcoal stripped FBS for 3 d then treated with dihydrotestosterone (DHT, 10 nM) for 4 hours (CST website) AR ChIP grade 17-10489 Millipore - validated by CHiP on DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Millipore website) All antibodies have been validated for WB on human cell lines in this study (clear band at the right Mw) AR ChIP antibodies have been validated using CHiP on LNCaP cells (KLK3 as a positive control) Eukaryotic cell lines Policy information about cell lines Cell line source(s) LNCaP, CWR22Res, VCap and C4-2 cells were purchased from ATCC.
Transfection:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: FKBP5 #12210 Cell Signaling Technology - validated by WB using HT-1080 and Hela cells (CST website) ACC #3676 Cell Signaling Technology - validated by WB using 3T3, 293 and various cell lines (CST website) ACLY #4332 Cell Signaling Technology - validated by WB using Hela, 3T3, C6 and COS cells (CST website) p-ACLY #4331 Cell Signaling Technology - validated by WB using PDGF-treated 3T3 cells (CST website) PKM1/2 #3190 Cell Signaling Technology - validated by WB using MCF-7, HepG2 and various cell lines (CST website) GLUT1 #12939 Cell Signaling Technology - validated by WB using RD, Huh6, 3T3 and A204 cells (CST website) AMPKa #2603 Cell Signaling Technology - validated by WB using 293, COS, PC12 cells and mouse brain extracts (CST website) p-AMPKa #2535 Cell Signaling Technology - validated by WB using oligomycin-treated C2C12 cells (CST website) BIP #3177 Cell Signaling Technology - validated by WB using A204, L929, U-87MG (CST website) DNAJC3 #2940 Cell Signaling Technology - validated by WB using 3T3, Hepal, bTC6 and NIT-1 cells (CST website) XBP1s #40435 Cell Signaling Technology - validated by WB using tunicamycin-treated 293T cells (CST website) CHOP #2895 Cell Signaling Technology - validated by WB using thapsigargin treated C6 and A204 cells (CST website) EPCAM #93790 Cell Signaling Technology - validated by WB using HCT116,HT-29 and various cell lines (CST website) ATF4 #11815 Cell Signaling Technology - validated by WB using tunicamycin-treated 293 and Hela cells (CST website) PARP #9532 Cell Signaling Technology - validated by WB using 293 PARP KO cells (CST website) DECR1 ab95965 Abcam - validated by WB using HepG2 cells (CST website) GPX4 ab125066 Abcam - validated by WB using mouse and rats testis lysates AR ChIP grade #5153 Cell Signaling Technology - validated by CHiP on LNCaP cells grown in phenol red free medium and 5% charcoal stripped FBS for 3 d then treated with dihydrotestosterone (DHT, 10 nM) for 4 hours (CST website) AR ChIP grade 17-10489 Millipore - validated by CHiP on DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Millipore website) All antibodies have been validated for WB on human cell lines in this study (clear band at the right Mw) AR ChIP antibodies have been validated using CHiP on LNCaP cells (KLK3 as a positive control) Eukaryotic cell lines Policy information about cell lines Cell line source(s) LNCaP, CWR22Res, VCap and C4-2 cells were purchased from ATCC.
Plasmid Preparation:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: FKBP5 #12210 Cell Signaling Technology - validated by WB using HT-1080 and Hela cells (CST website) ACC #3676 Cell Signaling Technology - validated by WB using 3T3, 293 and various cell lines (CST website) ACLY #4332 Cell Signaling Technology - validated by WB using Hela, 3T3, C6 and COS cells (CST website) p-ACLY #4331 Cell Signaling Technology - validated by WB using PDGF-treated 3T3 cells (CST website) PKM1/2 #3190 Cell Signaling Technology - validated by WB using MCF-7, HepG2 and various cell lines (CST website) GLUT1 #12939 Cell Signaling Technology - validated by WB using RD, Huh6, 3T3 and A204 cells (CST website) AMPKa #2603 Cell Signaling Technology - validated by WB using 293, COS, PC12 cells and mouse brain extracts (CST website) p-AMPKa #2535 Cell Signaling Technology - validated by WB using oligomycin-treated C2C12 cells (CST website) BIP #3177 Cell Signaling Technology - validated by WB using A204, L929, U-87MG (CST website) DNAJC3 #2940 Cell Signaling Technology - validated by WB using 3T3, Hepal, bTC6 and NIT-1 cells (CST website) XBP1s #40435 Cell Signaling Technology - validated by WB using tunicamycin-treated 293T cells (CST website) CHOP #2895 Cell Signaling Technology - validated by WB using thapsigargin treated C6 and A204 cells (CST website) EPCAM #93790 Cell Signaling Technology - validated by WB using HCT116,HT-29 and various cell lines (CST website) ATF4 #11815 Cell Signaling Technology - validated by WB using tunicamycin-treated 293 and Hela cells (CST website) PARP #9532 Cell Signaling Technology - validated by WB using 293 PARP KO cells (CST website) DECR1 ab95965 Abcam - validated by WB using HepG2 cells (CST website) GPX4 ab125066 Abcam - validated by WB using mouse and rats testis lysates AR ChIP grade #5153 Cell Signaling Technology - validated by CHiP on LNCaP cells grown in phenol red free medium and 5% charcoal stripped FBS for 3 d then treated with dihydrotestosterone (DHT, 10 nM) for 4 hours (CST website) AR ChIP grade 17-10489 Millipore - validated by CHiP on DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Millipore website) All antibodies have been validated for WB on human cell lines in this study (clear band at the right Mw) AR ChIP antibodies have been validated using CHiP on LNCaP cells (KLK3 as a positive control) Eukaryotic cell lines Policy information about cell lines Cell line source(s) LNCaP, CWR22Res, VCap and C4-2 cells were purchased from ATCC.
Positive Control:Article Title: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
Article Snippet: FKBP5 #12210 Cell Signaling Technology - validated by WB using HT-1080 and Hela cells (CST website) ACC #3676 Cell Signaling Technology - validated by WB using 3T3, 293 and various cell lines (CST website) ACLY #4332 Cell Signaling Technology - validated by WB using Hela, 3T3, C6 and COS cells (CST website) p-ACLY #4331 Cell Signaling Technology - validated by WB using PDGF-treated 3T3 cells (CST website) PKM1/2 #3190 Cell Signaling Technology - validated by WB using MCF-7, HepG2 and various cell lines (CST website) GLUT1 #12939 Cell Signaling Technology - validated by WB using RD, Huh6, 3T3 and A204 cells (CST website) AMPKa #2603 Cell Signaling Technology - validated by WB using 293, COS, PC12 cells and mouse brain extracts (CST website) p-AMPKa #2535 Cell Signaling Technology - validated by WB using oligomycin-treated C2C12 cells (CST website) BIP #3177 Cell Signaling Technology - validated by WB using A204, L929, U-87MG (CST website) DNAJC3 #2940 Cell Signaling Technology - validated by WB using 3T3, Hepal, bTC6 and NIT-1 cells (CST website) XBP1s #40435 Cell Signaling Technology - validated by WB using tunicamycin-treated 293T cells (CST website) CHOP #2895 Cell Signaling Technology - validated by WB using thapsigargin treated C6 and A204 cells (CST website) EPCAM #93790 Cell Signaling Technology - validated by WB using HCT116,HT-29 and various cell lines (CST website) ATF4 #11815 Cell Signaling Technology - validated by WB using tunicamycin-treated 293 and Hela cells (CST website) PARP #9532 Cell Signaling Technology - validated by WB using 293 PARP KO cells (CST website) DECR1 ab95965 Abcam - validated by WB using HepG2 cells (CST website) GPX4 ab125066 Abcam - validated by WB using mouse and rats testis lysates AR ChIP grade #5153 Cell Signaling Technology - validated by CHiP on LNCaP cells grown in phenol red free medium and 5% charcoal stripped FBS for 3 d then treated with dihydrotestosterone (DHT, 10 nM) for 4 hours (CST website) AR ChIP grade 17-10489 Millipore - validated by CHiP on DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Millipore website) All antibodies have been validated for WB on human cell lines in this study (clear band at the right Mw) AR ChIP antibodies have been validated using CHiP on LNCaP cells (KLK3 as a positive control) Eukaryotic cell lines Policy information about cell lines Cell line source(s) LNCaP, CWR22Res, VCap and C4-2 cells were purchased from ATCC.
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